Specimen Collection And Storage
1. Stool samples must be taken as soon as the symptoms appear.Viral particles decrease in number after one week.
2. Stool specimens should be collected in containers to the Rapid Rotavirus Antigen Test.
3. Specimens may be stored at 2-8℃ for 2 days without interfering with performance. For long-term storage of specimens, -20 ℃ or colder is recommended. Repeated freezing and thawing of specimens is not recommended and may cause erroneous results.
4. Unscrew the sample bottle, use the attached applicator stick attached on the cap to transfer small piece of stool into the sample
bottle containing specimen buffer.
5. Replace the stick in the bottle and tighten securely. Mix stool sample with the buffer thoroughly by skaing the bottle for a few
seconds.
Reagent Preparation
1.Bring all materials and specimens to room temperature (15– 30℃).
2.Remove the test card from the sealed foil pouch.
3.Hold the sample bottle upright with the tip point toward the direction away from the test performer, snap off the tip.
4.Hold the bottle in a vertical position over the sample well of the test card, deliver 3 drops (120 -150 μL) of diluted stool sample to the sample well.
5.Read the result between 15-20minutes.
Interpretation of Results
Positive: A distinct pink colored band appears on test line regions, in addition to a pink line on the control line region.
Negative: No line appears in the test line region. A distinct pink line shows on the control line region.
Invalid: The control line next to the test line does not become visible within 15 minutes after the addition of the sample.
